MODER (Medikal Ozon Oksijen Derneği)
ÖZOFAGUS YANIKLARINDA OZON
2010-02-12 at 12:12 am adminGATA ÇOCUK CERRAHİ VE FİZYOLOJİ AD ORTAK ÇALIŞMASI JOURNAL OF PEDİATRİC SURGERY’YE KABUL EDİLDİ. “ÖZOFAGUS YANIKLARINDA OZON TERAPİ”
The efficacy of ozone therapy in experimental caustic esophageal burn
Ahmet Guvena,, Gokhan Gundogdua, Serdar Sadirb, Turgut Topalb, Esra Erdoganc, Ahmet Korkmazb, İlhami Surera, Haluk Ozturka
a- Department of Pediatric Surgery, Gulhane Military Medical Academy, Etlik, Ankara 06017, Turkey
b- Department of Physiology, Gulhane Military Medical Academy, Etlik, Ankara 06017, Turkey
c- Department of Histology and Embrology, Gulhane Military Medical Academy, Etlik, Ankara 06017, Turkey
Received 22 October 2007; revised 11 January 2008; accepted 21 January 2008
Key words: Caustic Esophageal Burn;
Ozone therapy;
Antioxidant enzymes;
Lipid peroxidation;
Protein oxidation
Abstract
Introduction: Ozone has been proposed as an antioxidant enzyme activator, immunomodulator and cellular metabolic activator. This study was designed to investigate the efficacy of ozone therapy in the prevention of esophageal damage and stricture formation developed after esophageal caustic injuries in the rat.
Materials and Methods: Forty-five rats were allocated into three groups; sham-operated, un-treatment and treatment groups. Caustic esophageal burn was created by instilling 15% NaOH in the distal esophagus. The rats were left untreated or treated with 1 mg/kg/day ozone intraperitoneally. All rats were sacrificed at 28 days. Efficacy of the treatment was assessed by measuring the stenosis index (SI) and histopathologic damage score, and biochemically by determining tissue hydroxyproline content (HP), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) and protein carbonyl content (PCC) in esophageal homogenates.
Results: Whereas seven (47%) rats died in the un-treatment group, all rats in the sham-operated and the treatment group survived during the study. SI, the histopathologic damage score, was significantly lower in the ozone-therapy group than the un-treatment group. HP levels were significantly higher in the untreatment group than the group treated with ozone. Caustic esophageal burn increased MDA and PCC levels and also decreased SOD and GPx enzyme activities. In contrast, ozone therapy decreased the elevated MDA and PCC levels and also increased the reduced SOD and GPx enzyme activities.
Conclusion: Ozone has a preventive effect in the development of fibrosis by decreasing tissue damage and increasing the antioxidant enzyme activity in an experimental model of corrosive esophageal injury.
© 2008 Elsevier Inc. All rights reserved.
Ingestion of corrosive substances, leading to stricture formation as a late complication, remains a major health hazard in children [1,2]. This hazard remains despite aggressive accident prevention programs aimed at children and adult education, preventive labeling and packaging, and even legislation limiting the strength and availability of caustic substances [1,3].
Abbreviations: ROS, Reactive Oxygen Species; RNS, Reactive Nitrogen Species; SI, Stenosis Index (SI); MDA, Malondialdehyde; PCC, Protein Carbonyl Content; SOD, Superoxide Dismutase; GPx, Glutathione Peroxydase; HP, Hydroxyproline.
Corresponded author. Tel.: +90 312 3045483; fax: +90 312 3042150.
E-mail address: drahmetguven@yahoo.com (A. Guven).
www.elsevier.com/locate/jpedsurg
0022-3468/$ – see front matter © 2008 Elsevier Inc. All rights reserved.
doi:10.1016/j.jpedsurg.2008.01.064
Journal of Pediatric Surgery (2008) 43, 1679–1684